Correlation between antimicrobial resistance and biofilm formation capability among Klebsiella pneumoniae strains isolated from hospitalized patients in Iran

Abstract Background Klebsiella pneumoniae is a common cause of nosocomial infections. Antibiotic resistance and ability to form biofilm, as two key virulence factors K. , are involved in the persistence The purpose this study was investigate correlation between antimicrobial biofilm formation capability among strains isolated from hospitalized patients Iran. Methods Over 10-month period, total 100 non-duplicate were collected. susceptibility determined by Kirby–Bauer disk diffusion method according CLSI. Biofilm production assessed tissue culture plate method. Finally, polymerase chain reaction conducted detect four families carbapenemase: bla IMP VIM NDM OXA?48 ; associated genes: treC wza luxS confirming gene: rpoB. Results Most isolates resistant trimethoprim-sulfamethoxazole (52 %), cefotaxime (51 cefepime (43 ceftriaxone %). Among all isolates, 67 multidrug-resistant (MDR), 11 extensively drug-resistant (XDR). prevalence genes 7 5 28 %, respectively. results assay indicated that 75 (75 %) could produce only 25 (25 not able biofilm. these % formed fully established biofilms, 19 categorized moderately biofilm-producing, 31 weak non-biofilm-producers. former found be significantly higher than non-biofilm ( p < 0.05). Molecular distribution revealed 98 96 34 carried genes, Conclusions rise antibiotic biofilm-producer demonstrates serious concern about limited treatment options hospital settings. All data suggest fundamental actions introduction novel strategies for controlling biofilm-related infections essential.